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Braunii, increased growth temperatures led to a decrease in the relative content of more unsaturated intracellular fatty acids, especially trienoic species, while the composition of fatty acids secreted into a medium was unchanged (Sushchik et al. 2003). A decrease in cultivation temperature from 25 to 10°C resulted in an elevation of the relative proportion of oleate in the green alga Selenastrum capricornutum (McLarnonRiches et al. 1998). In contrast to the general expected increase in the proportion of fatty acid unsaturation levels, a decrease in linoleate and stearidonate (C18:4) at lower temperatures has been also shown in this alga (McLarnon-Riches et al.
1 Fatty acid distribution reported in TAG from selected algae species 2 Algal Lipids and Their Metabolism 23 24 promoter of B. braunii strain BOT-22 has been evaluated (Yonezawa et al. 2012). The growth and hydrocarbon accumulation were significantly higher in the cultures with 1 and 2% SCW. An addition of SCW also caused a shift in the hydrocarbon profile from C34H58 to C32H54 (Yonezawa et al. 2012). In addition, higher production of hydrocarbons in B. braunii Bot-144 (race B) has been achieved when it is grown under red light (Baba et al.
2003). Their data provided evidence for the operation of both of the biosynthetic pathways which had been described in plant and animal tissues previously. One reaction involved CDP-diacylglycerol and was catalyzed by PI synthase (CDP-diacylglycerol: myo-inositol 27 3-phosphatidyltransferase). In the second reaction (which did not in fact result in net PI formation), a free inositol was exchanged for an existing inositol headgroup. The major site of PI biosynthesis in C. reinhardtii was the microsomal (containing endoplasmic reticulum (ER)) fraction (Blouin et al.